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H2O 2.975 ml 0.5 M Tris-HCl, pH 6.8 1.25 ml 10% (w/v) SDS 0.05 ml Acrylamide/Bis-acrylamide (30%/0.8% w/v) 0.67 ml 10% (w/v) ammonium persulfate (AP) 0.05 ml TEMED 0.005 ml . Abstract Agarose gel electrophoresis is the most effective way of separating DNA fragments of varying sizes ranging from 100 bp to 25 kb1. Y., Costumbrado, J., Hsu, C. It is important to note that different forms of DNA move through the gel at different rates. Gel loading dye is typically made at 6X concentration (0.25% bromphenol blue, 0.25% xylene cyanol, 30% glycerol). Acrylamide % M.W. An example of a gel documentation system. Some suggestions: Go back to the last page Go to the home page .. Low melting agarose is generally used when the isolation of separated DNA fragments is desired.

It is important to use the same running buffer as the one used to prepare the gel. Note: Various factors affect the properties of the resulting gel. Lean more about SDS PAGE gel staining. Remove the lid. Determine the sizes of separated DNA fragments Protocol 1. Y., Kim, Y. Acylamide percentage 6% 8% 10% 12% 15% H2O 5.2ml 4.6ml 3.8ml 3.2ml 2.2ml Acrylamide/Bis-acrylamide (30%/0.8% w/v) 2ml 2.6ml 3.4ml 4ml 5ml 1.5M Tris(pH=8.8) 2.6ml 2.6ml 2.6ml 2.6ml 2.6ml 10% (w/v)SDS 0.1ml 0.1ml 0.1ml 0.1ml 0.1ml 10% (w/v) ammonium persulfate (AP) 100l 100l 100l 100l 100l TEMED 10l 10l 10l 10l 10l . (Approximately vol.

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